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Background: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.

Host species/Isotype: Rabbit/IgG

Species reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit

Immunogen: KLH conjugated synthetic peptide derived from human CK5.

 Storage instructions: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Concentration: 1mg/ml

Purity: affinity purified by Protein A

Clonality: Polyclonal

Molecular weight: 64KDa

Tested applications:

WB=1:500-2000

ELISA=1:500-1000

IHC-P=1:100-500

IHC-F=1:100-500

Flow-Cyt=1μg/Test

IF=1:100-500