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商品編號(hào)


53011



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分類(lèi)


Methyltransferase(s)



Size

96 reactions

商品信息













Description:
The SUV4-20H1 Chemiluminescent Assay Kit is designed to measure SUV4-20H1 activity for screening and profiling applications. The key to the SUV4-20H1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.




Synonym(s):
Suppressor Of Variegation 4-20 Homolog 1 , KMT5B, SUV420H1




Supplied As:
Kit comes in a convenient format, with 96-well plate precoated with histone H4 substrate, reagents, and purified SUV4-20H1 enzyme for 96 enzyme reactions.




Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt




Format:

COMPONENTS





Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.




Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.




Reference(s):
Dillon S.C., et al. 2005. Genome
BIOLOG
y 6:227.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Methyltransferase




Product Type:
Assay Kit




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