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商品編號


BL21-100



品牌


MCLab



公司


MCLAB.Inc



公司分類


Competent Cells



Description

10x100μl (10 tubes), >1x10?


Size

1 Ea

商品信息

Chemically competent
E. coli
cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.




Description:
Chemically competent
E. coli
cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.


Application:

Ideal for expressing proteins in
E. coli.


Recommended torage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Recommended reaction conditions:

Mix DNA with competent cells

Let stand on bench for 5 minutes

Direct plate


Genotype:
E. coli
B F-
dcm ompT hsdS
(rB- mB-) gal [malB+]K-12(λS)
Reference:
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.


Competent Cell Type:
Chemically


Transformation Efficiency (cfu/μg):
>1x10
8


Blue-Whiet Screening:
No


Strain:
B


Reduces Recombination:
No


Cloning Methylated DNA:
No


Improves Plasmid Quality:
No


Preparing Unmethylated DNA:
Not suitable


T1 Phage Resistant:
No


RecA Deficient:
No