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商品編號


BS-100



品牌


MCLab



公司


MCLAB.Inc



公司分類


Competent Cells



Description

10x100μl (10 tubes), >1x10?


Size

1 Ea

商品信息

High yields, high efficiency, and no heat shock necessary.




Description
High yields, high efficiency, and no heat shock necessary.


Application

Ideal for expressing proteins in
E. coli.

BL21(DE3) cells carry the lam
BD
a DE3 lysogen.


Recommended torage condition
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Recommended reaction conditions

Mix DNA with competent cells

Let stand on bench for 5 minutes

Direct plate


Genotype
F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Reference
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.


Competent Cell Type:
Chemically


Transformation Efficiency (cfu/μg):
>1x10
8


Blue-Whiet Screening:
No


Strain:
B


Reduces Recombination:
No


Cloning Methylated DNA:
No


Improves Plasmid Quality:
No


Preparing Unmethylated DNA:
Not suitable


T1 Phage Resistant:
No


RecA Deficient
:
No