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商品編號


3440-005-01



品牌


Trevigen



公司


Trevigen, Inc



公司分類


Extracellular Matrix Proteins



Size

1 ml, 20 ml

商品信息

Description

Cultrex? Rat?Collagen I?is purified from rat tail tendons?and?is provided at a high concentration that is capable of polymerizing to form a?hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37? C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.

Cultrex? Rat?Collagen I?is a purified stromal ECM protein?that has been developed, produced and qualified for general cell culture applications.

Quality control specifications:


No bacterial or fungal growth detected after incubation at 37°C for 14 days following
USP
sterility testing guidelines.

Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.

No mycoplasma contamination detected by PCR.

Protein concentration of 5 mg/ml.

Provided in?20 mM Acetic Acid, pH 3.4 – 3.6.

Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.

BIOLOG
ical activity determined for each lot:

Cell Attachment: Tested for the
ABI
lity to promote cell attachment and spre
ADI
ng of HT1080 human fibrosarcoma cells.





Glycosylation at Asn211 Regulates the Activation State of the Discoidin Domain Receptor 1 (DDR1)
Fu, H.L., Valiathan, R.R., Payne, L., Kumarasiri, M., Mahasenan, K.V., Mobashery, S., Huang, P. and Fridman, R.
J. Biol. Chem., Mar 2014; 289: 9275 – 9287.
http://www.jbc.org/cgi/content/abstract/289/13/9275

RPTPα controls epithelial adherens junctions, linking E-cadherin engagement to c-Src-mediated phosphorylation of cortactin
Marta Truffi, Véronique Dubreuil, Xuan Liang, Nathalie Vacaresse, F
ABI
enne Nigon, Siew Ping Han, Alpha S. Yap, Guillermo A. Gomez, and Jan Sap
J. Cell Sci., Jun 2014; 127: 2420 – 2432.
http://jcs.
BIOLOG
ists.org/cgi/content/abstract/127/11/2420

Biphasic Modulation of Paracellular Claudin-5 Expression in Mouse Brain Endothelial Cells Is Mediated through the Phosphoinositide-3-Kinase/AKT Pathway
Ryan B. Camire, Holly J. Beaulac, Stephanie A. Brule, Annie I. McGregor, Emily E. Lauria, and Colin L. Willis
J. Pharmacol. Exp. Ther., Oct 2014; 351: 654 – 662.
http://jpet.aspetjournals.org/cgi/content/abstract/351/3/654

DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity
Qian Zhang, Christopher G. Dove, Jyh Liang Hor, Heardley M. Murdock, Dara M. Strauss-Albee, Jordan A. Garcia, Judith N. Mandl, Rachael A. Grodick, Huie Jing, Devon B. Chandler-Brown, Timothy E. Lenardo, Greg Crawford, Helen F. Matthews, Alexandra F. Freeman, Richard J. Cornall, Ronald N. Germain, Scott N. Mueller, and Helen C. Su
J. Exp. Med., Nov 2014; 10.1084/jem.20141307.
http://jem.rupress.org/cgi/content/abstract/jem.20141307v1

“Maspin Expression in Prostate Tumor Cells Averts Stemness and
Stratifies Drug Sensitivity”
“M. Margarida Bernardo, Alexander Kaplun, Sijana H. Dzinic, Xiaohua Li, Jonathan Irish, Adelina Mujagic,
Benjamin Jakupovic, Jessica B. Back, Eric Van Buren, Xiang Han, Ivory Dean, Yong Q. Chen,
ELISA
beth
Heath, Wael Sakr, and Shijie Sheng”
Cancer Res., Sep 2015; 75: 3970 – 3979.
http://cancerres.aacrjournals.org/cgi/content/abstract/75/18/3970

Mesencephalic Astrocyte–Derived Neurotrophic Factor as a Urine Bio
Marker
for Endoplasmic Reticulum Stress–Related Kidney Diseases
Yeawon Kim, Heedoo Lee, Scott R. Manson, Maria Lindahl, Bradley Evans, Jeffrey H. Miner, Fumihiko Urano, and Ying Maggie Chen
J. Am. Soc. Nephrol., Mar 2016; 10.1681/ASN.2014100986
http://jasn.asnjournals.org/cgi/content/abstract/ASN.2014100986v1